Coding

Part:BBa_K4220011:Design

Designed by: Xiaoya Liu   Group: iGEM22_NEU_CHINA   (2022-09-25)


alpha factor sp-V5-RTAC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1407
    Illegal BamHI site found at 2288
    Illegal XhoI site found at 2279
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 471
    Illegal XbaI site found at 1404
    Illegal PstI site found at 321
    Illegal PstI site found at 760
    Illegal PstI site found at 2132
    Illegal AgeI site found at 2391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 283
    Illegal BsaI site found at 1604
    Illegal BsaI.rc site found at 1292


Design Notes

To express RTAC in the yeast, signal peptide will be replaced with yeast alpha factor signaling peptide.


Source

alpha factor sp: yeast RTAC: mammalian

References